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Publications
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Ligon AH, Morton CC, Bieber FR, Fletcher JA, Giersch ABS, Lee C, Sandstrom M, SWeremowicz S, Xiao S, Dal Cin P.
Reporting of Diagnostic Cytogenetic Results. In: Dracopoli NC, Haines JL, Korf BR, Morton CC, Seidman CE, Seidman JG, Smith DR, editors. Current Protocols in Human Genetics, John Wiley and Sons; 2004. p.A.1D.1-28
>>abstract
This appendix, developed by the staff at the Clinical Cytogenetics Laboratory at the Brigham and Women's Hospital, provides a comprehensive list of the facilities' current "macros" or standardized statements, used to facilitate reporting of cytogenetic results. These are provided as a reference for other laboratories. The statements are organized under the general categories of constitutional or acquired abnormalities and subdivided into analysis type (GTG-banding or FISH). Multi-specimen usage macros are included that can be applied to two or more specimen types.
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Lee C, Smith A.
Molecular cytogenetic methodologies and a BAC probe panel resource for genomic analyses in the zebrafish. In: Detrich HW, Westerfield M, Zon LI, editors. Methods in Cell Biology, 77(2nd), Elsevier; 2004. p.241-254
>>abstract
In an effort to establish molecular cytogenetic tools for zebrafish genomic analyses, we have developed a first generation zebrafish BAC probe panel using genetically-positioned BAC clones. The following methods were used to chromosomally map the BAC probes in this panel. The same methods may also be used in experiments to assess for genomic imbalances, ploidy and genomic instability in the zebrafish.
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Lee C, Rens W, Yang F.
Multicolor fluorescence in situ hybridization (FISH) approaches for simultaneous analysis of the entire human genome. In: Dracopoli NC, Haines JL, Korf BR, Morton CC, Seidman CE, Seidman JG, Smith DR, editors. Current Protocols in Human Genetics, John Wiley and Sons; 2000. p.4.9.1-11
>>abstract
"Chromosome" comes from the Greek meaning "colored body". The term was first used by Waldeyer in 1888 to describe the dark-staining bodies that he saw in the nucleus. A hundred years later, chromosomes truly became "colored bodies" when Pinkel et al. (1988) and Cremer et al. (1988) performed the first human chromosome fluorescence in situ hybridization (FISH) painting experiments, detecting whole chromosomes in single fluorescent colors. When prior indications were not available, searching for chromosomal aberrations by single color FISH methods usually meant applying numerous probes to the same sample in a laborious and time-consuming fashion. Multicolor FISH protocols made such tasks easier by allowing the simultaneous visualization of several chromosomal targets in different colors. Further advances in molecular cytogenetics have now made it possible to examine efficiently the entire human genome in a single experiment. These technologies are especially useful when specimen material is limited and numerous complex chromosomal rearrangements are involved. Here we present an overview of the multicolor FISH approaches currently available for genome-wide analyses.
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