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Publications
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Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling circle amplification and fluoescence in situ hybridization.
Appl Environ Microbiol. 2007; 73:2324-2328
>>abstract
An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.
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Fluorescence-based detection of short DNA sequences under non-denaturing conditions.
Bioorganic & Medicinal Chemistry. 2007; :
>>abstract
The ability of peptide nucleic acid (PNA) to open up duplex DNA in a highly sequence-specific manner makes it possible to detect short DNA sequences on the background of or within genomic DNA under non-denaturing conditions.
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Freeman JL, Adeniyi A, Banerjee R, Dallaire S, Maquire SF, Chi J, Ng BL, Zepeda C, Scott CE, Humphray S, Rogers J, Zhou Y, Zon LI, Carter NP, Yang F, Lee C
Definition of the zebrafish genome using flow cytometry and cytogenetic mapping.
BMC Genomics. 2007; 8:195
>>abstract
The zebrafish (Danio rerio) is an important vertebrate model organism system for biomedical research. The syntenic conservation between the zebrafish and human genome allows one to investigate the function of human genes using the zebrafish model. To facilitate analysis of the zebrafish genome, genetic maps have been constructed and sequence annotation of a reference zebrafish genome is ongoing. However, the duplicative nature of teleost genomes, including the zebrafish, complicates accurate assembly and annotation of a representative genome sequence. Cytogenetic approaches provide “anchors” that can be integrated with accumulating genomic data.
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Shepard JL, Amatruda JF, Finkelstein D, Ziai J, Finley KR, Stern HM, Chiang, K, Hersey C, Barut B, Freeman JL, Lee C, Glickman JN, Kutok JL, Aster JC, Zon LI
A mutaton in separase causes genome instability and increased susceptibility to eptithelial cancer.
Genes Dev. 2007; 21:55-9
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Proper chromosome segregation is essential for maintenance of genomic integrity and instability resulting from failure of this process may contribute to cancer. Here, we demonstrate that a mutation in the mitotic regulator separase is responsible for the cell cycle defects seen in the zebrafish mutant, cease&desist (cds). Analysis of cds homozygous mutant embryos reveals high levels of polyploidy and aneuploidy, spindle defects, and a mitotic exit delay. Carcinogenesis studies demonstrated that cds heterozygous adults have a shift in tumor spectrum with an eightfold increase in the percentage of fish bearing epithelial tumors, indicating that separase is a tumor suppressor gene in vertebrates. These data strongly support a conserved cross-species role for mitotic checkpoint genes in genetic stability and epithelial carcinogenesis.
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Structural variation in the human genome: The impact of copy number variants on clinical diagnosis.
Genet Med. 2007; 9:600-606
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Over the past few years, the application of whole-genome scanning array technologies has catalyzed the appreciation of a new form of submicroscopic genomic imbalances, referred to as copy number variants. Copy number variants contribute substantially to genetic diversity and result from gains and losses of genomic regions that are 1000 base pairs in size or larger, sometimes encompassing millions of bases of contiguous DNA sequences. As genome-wide scanning techniques become more widely used in diagnostic laboratories, a major challenge is how to accurately interpret which submicroscopic genomic imbalances are pathogenic in nature and which are benign. Herein, we review the literature from the past 3 years on this new source of genomic variability and comment on factors that should be considered when trying to differentiate between a pathogenic and a benign copy number variant.
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Copy number variations and clinical cytogenetic diagnosis of constitutional disorders.
Nat Genet. 2007; 39:S48-S54
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The recent appreciation of widespread copy number variation in the genomes of healthy human beings has presented a significant challenge to clinical cytogeneticists who wish to use genome-wide array comparative genomic hybridization (CGH) assays for clinical diagnostic purposes. Clinical cytogeneticists need to differentiate between copy number variants (CNVs) that are likely to be pathogenic and CNVs that are less likely to contribute to an affected individual’s clinical presentation. Unfortunately, our knowledge of the phenotypic effects of most CNVs is minimal, leading to the classification of many CNVs as genomic imbalances of unknown clinical significance. This has caused many laboratories to resist the use of higher-resolution genome-wide array CGH assays for clinical purposes. Ironically, the accumulation and annotation of such array CGH data can lead to the rapid identification of pathogenic CNVs and the definition of new genomic syndromes that, in turn, are useful for accurate clinical genetic diagnoses.
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Challenges and standards in integrating surveys of structural variation.
Nat Genet. 2007; 39:S7-S15
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There has been an explosion of data describing newly
recognized structural variants in the human genome. In the
flurry of reporting, there has been no standard approach to
collecting the data, assessing its quality or describing identified features. This risks becoming a rampant problem, in particular with respect to surveys of copy number variation and their application to disease studies. Here, we consider the challenges in characterizing and documenting genomic structural variants. From this, we derive recommendations for standards to be adopted, with the aim of ensuring the accurate presentation of this form of genetic variation to facilitate ongoing research.
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Perry GH, Dominy NJ, Claw KG, Lee AS, Fiegler H, Redon R, Werner J, Villanea FA, Mountain JL, Misra R, Carter NP, Lee C*, Stone AC*.
Diet and the evolution of human gene copy number variation.
Nat Genet. 2007; :
>>abstract
Starch consumption is a prominent characteristic of agricultural societies and hunter-gatherers in arid environments. In contrast, rainforest and circum-arctic huntergatherers and some pastoralists consume much less starch1–3. This behavioral variation raises the possibility that different selective pressures have acted on amylase, the enzyme responsible for starch hydrolysis4. We found that copy number of the salivary amylase gene (AMY1) is correlated positively with salivary amylase protein level and that individuals from populations with high-starch diets have, on average, more AMY1 copies than those with traditionally low-starch diets. Comparisons with other loci in a subset of these populations suggest that the extent of AMY1 copy number differentiation is highly unusual. This example of positive selection on a copy number–variable gene is, to our knowledge, one of the first discovered in the human genome. Higher AMY1 copy numbers and protein levels probably improve the digestion of starchy foods and may buffer against the fitness-reducing effects of intestinal disease.
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Eichler EE, Nickerson DA, Altshuler D, Bowcock AM, Brooks LD, Carter NP, Church DM, Felsenfeld A, Lee C, Lupski JR, Mullikin JC, Pritchard JK, Sebat J, Sherry ST, Smith D, Waterson RH
Completing the map of human genetic variation.
Nature. 2007; 447:161-165
>>abstract
Large-scale studies of human genetic variation have focused largely on understanding the pattern and nature of single-nucleotide differences within the human genome. Recent studies that have identified larger polymorphisms,
such as insertions, deletions and inversions, emphasize the value of investing in more comprehensive and systematic studies of human structural genetic variation. We describe a
community resource project recently launched by the National Human Genome Research Institute (NHGRI) to sequence large-insert clones from many individuals, systematically discovering and resolving these complex variants at the DNA sequence level. The project includes the discovery of variants through development of clone resources, sequence resolution of variants, and accurate typing of variants in individuals of African, European or Asian ancestry. Sequence resolution of both single-nucleotide and larger-scale genomic variants will improve our picture of natural variation in human populations and
will enhance our ability to link genetics and human health.
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Demichelis F, Fall K, Perner S, Andren O, Schmidt F, Setlur SR, Hoshida Y, Mosquera JM, Pawitan Y, Lee C, Adami HO, Mucci LA, Kantoff PW, Andersson SO, Chinnaiyan AM, Johansson JE, Rubin MA
TMPRSS2:ERG gene fusion associated with lethal prostate cancer in a watchful waiting cohort..
Oncogene. 2007; 26:4596-4599
>>abstract
The identification of the TMPRSS2:ERG fusion in prostate cancer suggests that distinct molecular subtypes may define risk for disease progression. In surgical series, TMPRSS2:ERG fusion was identified in 50% of the tumors. Here, we report on a population-based cohort of men with localized prostate cancers followed by expectant (watchful waiting) therapy with 15% (17/111) TMPRSS2:ERG fusion. We identified a statistically significant association between TMPRSS2:ERG fusion and prostate cancer specific death (cumulative incidence ratio¼2.7, Po0.01, 95% confidence interval¼1.3–5.8). Quantitative reverse-transcription–polymerase chain reaction demonstrated high estrogen-regulated gene (ERG) expression to be associated with TMPRSS2:ERG fusion (Po0.005). These data suggest that TMPRSS2:ERG fusion prostate cancers may have a more aggressive phenotype, possibly mediated through increased ERG expression.
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Ligon AH, Morton CC, Bieber FR, Fletcher JA, Giersch ABS, Lee C, Sandstrom M, SWeremowicz S, Xiao S, Dal Cin P.
Reporting of Diagnostic Cytogenetic Results. In: Dracopoli NC, Haines JL, Korf BR, Morton CC, Seidman CE, Seidman JG, Smith DR, editors. Current Protocols in Human Genetics, John Wiley and Sons; 2004. p.A.1D.1-28
>>abstract
This appendix, developed by the staff at the Clinical Cytogenetics Laboratory at the Brigham and Women's Hospital, provides a comprehensive list of the facilities' current "macros" or standardized statements, used to facilitate reporting of cytogenetic results. These are provided as a reference for other laboratories. The statements are organized under the general categories of constitutional or acquired abnormalities and subdivided into analysis type (GTG-banding or FISH). Multi-specimen usage macros are included that can be applied to two or more specimen types.
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Lee C, Smith A.
Molecular cytogenetic methodologies and a BAC probe panel resource for genomic analyses in the zebrafish. In: Detrich HW, Westerfield M, Zon LI, editors. Methods in Cell Biology, 77(2nd), Elsevier; 2004. p.241-254
>>abstract
In an effort to establish molecular cytogenetic tools for zebrafish genomic analyses, we have developed a first generation zebrafish BAC probe panel using genetically-positioned BAC clones. The following methods were used to chromosomally map the BAC probes in this panel. The same methods may also be used in experiments to assess for genomic imbalances, ploidy and genomic instability in the zebrafish.
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Lee C, Rens W, Yang F.
Multicolor fluorescence in situ hybridization (FISH) approaches for simultaneous analysis of the entire human genome. In: Dracopoli NC, Haines JL, Korf BR, Morton CC, Seidman CE, Seidman JG, Smith DR, editors. Current Protocols in Human Genetics, John Wiley and Sons; 2000. p.4.9.1-11
>>abstract
"Chromosome" comes from the Greek meaning "colored body". The term was first used by Waldeyer in 1888 to describe the dark-staining bodies that he saw in the nucleus. A hundred years later, chromosomes truly became "colored bodies" when Pinkel et al. (1988) and Cremer et al. (1988) performed the first human chromosome fluorescence in situ hybridization (FISH) painting experiments, detecting whole chromosomes in single fluorescent colors. When prior indications were not available, searching for chromosomal aberrations by single color FISH methods usually meant applying numerous probes to the same sample in a laborious and time-consuming fashion. Multicolor FISH protocols made such tasks easier by allowing the simultaneous visualization of several chromosomal targets in different colors. Further advances in molecular cytogenetics have now made it possible to examine efficiently the entire human genome in a single experiment. These technologies are especially useful when specimen material is limited and numerous complex chromosomal rearrangements are involved. Here we present an overview of the multicolor FISH approaches currently available for genome-wide analyses.
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Charles Lee
Two mammalian centromeric satellite DNA families (1996). Department of Medical Sciences, University of Alberta
Edmonton, Alberta, Canada.
>>abstract
Two mammalian centromeric satellite DNA families
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Charles Lee
Tandemly repetitive DNA in the karyotypic and phylogenetic evolution of Cervidae species (1993). Department of Laboratory Medicine and Pathology, University of Alberta
Edmonton, Alberta, Canada.
>>abstract
Tandemly repetitive DNA in the karyotypic and phylogenetic evolution of Cervidae species
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